ANALYTICAL TEST PROCEDURE OF CITRIC ACID ANHYDROUS

 

MATERIAL NAME: CITRIC ACID ANHYDROUS
Molecular Formula: C6H8O7	Formula Weight: 192.13	Equivalent Weight: 64.03

 

SL NO.	Test Parameters	Specification
1.	Physical State	Colorless crystal or White Crystalline Powder
2.	Identification	A neutral solution of citric acid is boiled with HgSO4 TS (5 g in 80 ml of water), then filtered. Few drops of KMnO4 TS (10 g/l) is added to the filtrate, a white precipitate is formed.
3.	Clarity	10 % W/W Solution Should Be Clear Compared With Existing Good One
4.	Loss on drying	NMT 1.0% w/w at 105°C for 15 minutes, use 2 g
5.	Assay (%)	99.5 % to 100.5 % as C6H8O7 calculated on anhydrous basis.

 

METHOD OF ASSAY ANALYSIS

1. Place About 0.55 g Of Anhydrous Citric Acid In A Tarred Conical Flask &Weigh Accurately. 2. Dissolve In 50ml Of Water. 3. Add 0.5ml Of Phenolphthalein TS. 4. Titrate With 1N Sodium Hydroxide VS. 5. End Point Is Determined When Just Pink Color Appeared.

 

CALCULATION OF ASSAY PERCENTAGE

Each ml Of 1N Sodium Hydroxide Is Equivalent To 64.03 mg Of C6H8O7 Or BR × Normality Of NaOH ×Equivalent Weight Of Citric Acid(64.03)×100 Sample Weight×1000

 

Precautions:

1.         Standardize Sodium Hydroxide Solution Before Titration.

2.         Weighing Should Be Accurate

REFERENCE: USP-29